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Fluorescence Study Of Dyes
Beginning of essay
1. Introduction
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse th ....
Middle of essay
....Sodium fluorescein
C = 2.425 x10-5 mol dm -3
Here max was 500.5 nm at an absorbance of 2.0922
Summary of the results obtained.
Dye
max (nm)
max (m2 mol-1)
(m2 mol-1)
' (m2 mol-1)
D (Coumarin)
376.5
1.82 x 109
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1.25 x 106
A (Fluorescein)
500.5
5.17 x 107
1.41 x 106
8 x 106
2.2. Fluorimetry
a) Perylene standard
/nm I
Emission Spectrum 438 4.063 EX = 434nm
467 2.620
Excitation Spectrum 410 2.755 EX = 438n.... |
| Number of words: 815 |
Approximate pages: 3 |
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