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Determination Of An Unknown Amino Acid From Titration

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Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96, and the pKa’s found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were found to be 2.10, 4.07, and 9.47. The molecular weight was calculat ....

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....e pH versus the volume of titrant used. In the case of amino acids, the titrant will be both an acid and a base. The acid is a useful tool because it is able to add a proton to the amine group (see fig. 1). Likewise the base allows for removal of the proton from the carboxyl group by the addition of hydroxide. The addition of the strong acid or base does not necessarily yield a drastic jump in pH. The acid or base added is unable to contribute to the pH of the solution because the protons a....
Number of words: 1634 Approximate pages: 6

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