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Green Fluorescent Protein Purification
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Green Fluorescent Protein Purification
Green Fluorescent Protein (GFP) is a relatively new biochemical tool with a wide range of applications in current research. The natural function of GFP is to facilitate the bioluminescence of jellyfish and other coelenterates. Aequorea GFP (from jellyfish) c ....
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....valent hypothesis of this experiment was that T3 is essential in directing TR proteins to the nucleus of the cell where the proteins bind to DNA, regulating thyroid activity.
GFP was fused to the amino terminal because that region is not involved in TRb1’s hormone and transcriptional activity whereas the COOH-terminal region is essential to this protein’s activities. In vitro studies showed that the addition of GFP to TRb1 did not affect TRb1’s ability to bind to T3. Numerous other studies rei.... |
| Number of words: 1280 |
Approximate pages: 5 |
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